01 Product Description
賽多利斯腺病毒純化套組Sartorius Vivapure® AdenoPACK™
重組腺病毒載體(Recombinant adenovirus vectors)是目前效率最好的基因轉殖系統之一,然而傳統的 CsCl 載體回收法費時而冗長,Vivapure® AdenoPACK™ Kit 則採用最新的濾膜技術與試劑系統,將 digestion、virus binding、washing、elution、concentration 等步驟在 2 小時之內執行完畢,最多可於 HEK293 細胞中回收達3 x 1013 的病毒量,回收率為 65-80%,最終體積1 ml。
Fast and Easy to use Kits for Adenovirus Purification Recombinant adenovirus vectors are the preferred method for a wide range of gene delivery applications. The AdenoPACK™ adenovirus purification and concentration kits offer researchers who need to recover up to 3 x 1013 purified recombinant adenovirus particles for in-vitro transfection a fast, safe and easy to use solution. The kits include all reagents and devices necessary for clarification, purification and concentration of adenovirus type 5 from HEK293 cell cultures in only two hours. These straight forward kits replace time-consuming and labor-intensive 48 hour CsCl density gradients.
系列產品:AdenoPACK™ 20 / AdenoPACK™ 100 / AdenoPACK™ 500
賽多利斯腺病毒純化套組Sartorius Vivapure® AdenoPACK™產品列表
純化方法 | 處理時間 | 洗脫液 | 復甦 | 病毒顆粒 |
---|---|---|---|---|
AdenoPACK TM 20 / 20ml培養物中 | 1小時 | 1 mL | 65-70% | 1 x 1011-12 |
AdenoPACK™ 100 / 60 ml culture | 1-2小時 | 1 mL | 65% | 1-3x1012 |
AdenoPACK™ 100 / 200 ml culture | 2小時 | 1 mL | 80% | 1 x 1013 |
AdenoPACK™ 100 RT ***/ 200 ml culture | 2小時 | 1 mL | 80% | 1 x 1013 |
AdenoPACK™ 500 / 500 ml culture | 1-2小時 | 1 mL | 80% | 1-3x1013 |
套件規格AdenoPACK™ 20 | ||||
樣本大小 | 最多6×20毫升的腺病毒上清液 | - | - | - |
恢復的病毒顆粒(VP) | 通常每20 ml製劑最多1 x 1012 | - | - | - |
VP/IU | 20 - 50 | - | - | - |
處理時間 | 通常為1小時 | - | - | - |
內毒素水平 | <0.025 EU / mL | - | - | - |
Kit Specifications (AdenoPACK™ 100) | ||||
樣本大小 | 20至200毫升的腺病毒上清液 | - | - | - |
恢復的病毒顆粒(VP) | 通常每20 ml製劑最多1 x 1013 | - | - | - |
VP/IU | 20 - 50 | - | - | - |
處理時間 | 通常為2小時 | - | - | - |
內毒素水平 | <0.025 EU / mL | - | - | - |
Kit Specifications (AdenoPACK™ 500) | ||||
樣本大小 | 多達500毫升的腺病毒上清液 | - | - | - |
恢復的病毒顆粒(VP) | 通常每20 ml製劑最多3 x 1013 | - | - | - |
VP/IU | 20 - 50 | - | - | - |
處理時間 | 通常為2小時 | - | - | - |
內毒素水平 | <0.025 EU / mL | - | - | - |