{"id":14389,"date":"2020-10-16T16:57:53","date_gmt":"2020-10-16T08:57:53","guid":{"rendered":"https:\/\/www.cmp-micro.com\/ch\/?p=14389"},"modified":"2024-07-08T11:59:42","modified_gmt":"2024-07-08T03:59:42","slug":"20201610","status":"publish","type":"post","link":"https:\/\/www.cmp-micro.com\/ch\/20201610\/","title":{"rendered":"\u4fdd\u5b58\u578b\u8207\u53bb\u6d3b\u5316\u578b\u75c5\u6bd2\u6aa2\u9ad4\u904b\u9001\u57f9\u990a\u57fa\u7684\u6548\u80fd\u8a55\u4f30"},"content":{"rendered":"

\"\"<\/p>\n

\u75c5\u6bd2\u6aa2\u9ad4\u904b\u9001\u57f9\u990a\u57fa(transport media)\u7684\u4e3b\u8981\u529f\u80fd\u662f\u4fdd\u5b58\u6aa2\u9ad4\u4e2d\u75c5\u539f\u9ad4\u7684\u6d3b\u6027\u3001\u6297\u539f\u53ca\u6838\u9178 \u5176\u53ef\u907f\u514d\u6aa2\u9ad4\u5728\u904b\u9001\u904e\u7a0b\u4e2d\u53d7\u5230\u6c59\u67d3\u3002\u7531\u65bc\u65b0\u51a0\u75c5\u6bd2(SARS-CoV-2)\u80ba\u708e\u75ab\u60c5(COVID-19)\u7684\u7206\u767c \u5176\u8a3a\u65b7\u4fc2\u4ee5\u62ed\u5b50(swab)\u63a1\u96c6\u547c\u5438\u9053\u6aa2\u9ad4\u9032\u884c\u5206\u5b50\u6aa2\u6e2c\u53ca\/\u6216\u990a\u6b96\u7d30\u80de\u63a5\u7a2e\u3002\u555f\u65b0\u751f\u7269\u79d1\u6280\u6709\u9650\u516c\u53f8\u65bc 2020 \u5e74\u4e0a\u5e02\u5169\u7a2e\u901a\u7528\u578b\u75c5\u6bd2\u6aa2\u9ad4\u904b\u9001\u57f9\u990a\u88dd\u7f6e \u4e00\u70ba\u4fdd\u5b58\u578bCMPTM viral transport medium w\/oropharyngeal and\/or nasopharyngeal flocked swab (VTM) \u53ef P2+ \u6216 P3 \u7b49\u7d1a\u6aa2\u9a57\u5ba4\u5206 \u75c5\u6bd2\u53ca\u9032\u884c\u5206\u5b50\u6aa2\u6e2c \u4e00\u70ba\u53bb\u6d3b\u5316\u578b CMPTM inactivated viral transport medium w\/oropharyngeal and\/or nasopharyngeal flocked swab (I-VTM) \u50c5\u53ef\u6aa2\u9a57\u5ba4\u9032\u884c\u75c5\u6bd2\u4e4b\u5206\u5b50\u6aa2\u6e2c\u3002\u70ba\u4e86\u9a57\u8b49\u6b64\u5169\u7a2e\u75c5\u6bd2\u6aa2\u9ad4\u904b\u9001\u57f9\u990a\u57fa\u7684\u6548\u80fd \u543e\u7b49\u6839\u64da CLSI (Clinical and Laboratory Standards Institute) \u4e4b \u75c5 \u6bd2 \u904b \u9001 \u4e4b M40-A2 (2014) SARS-CoV-2 \u540c RNA \u75c5\u6bd2\u7684\u8178\u75c5\u6bd2 71 \u578b(Enteroviruses 71, EV 71)\u9032\u884c\u6548\u80fd\u8a55\u4f30\u3002\u6e2c\u8a66\u6642\u5206\u5225\u5c07 103 PFU (plaque forming unit)\u75c5\u6bd2\u91cf\u63a5\u7a2e\u81f3 VTM \u6216 I-VTM \u4e2d \u4e26\u6a21\u64ec\u75c5\u6bd2\u6aa2\u9ad4\u7684\u904b\u9001\u53ca\u4fdd\u5b58\u689d\u4ef6 \u5206\u5225\u5c07\u5169\u7a2e\u88dd\u7f6e\u7684\u904b\u9001\u57f9\u990a\u57fa\u7f6e\u65bc4C\u3001-80C\u53ca\u5ba4\u6eab \u4e26\u5206\u5225\u8caf\u5b58\u4e0d\u540c\u7684\u6307\u5b9a\u6642\u9593\u9ede\uff08\u5728 4C\u4fdd\u5b58 6\u300124\u300148\u300172 \u53ca 96 \u5c0f\u6642 \u800c\u5728-80C\u53ca\u5ba4\u6eab\u4fdd\u5b58 48\u300172 \u53ca 96 \u5c0f\u6642\u3002\u6b64\u5916 I-VTM \u5728\u5404\u6e2c\u8a66\u6eab\u5ea6\u984d\u5916\u4fdd\u5b58 10 \u5206\u9418\u300130 \u5206\u9418\u30011\u30012\u30013 \u53ca 4 \u5c0f\u6642\uff09\u5f8c\u5c07VTM \u6216I-VTM \u7684EV 71 \u5206\u5225 \u7a2e\u65bc \u7d30\u80de(human rhabdomyosarcoma cell line, RD cell) \u4e2d \u63a5 \u5728 5% CO2 \u57f9\u990a \u4e2d \u57f9\u990a 48-72 \u5c0f\u6642\u5f8c RD cell \u7684\u7d30\u80de\u75c5 \u6548\u61c9(cytopathic effects, CPE)\u4ee5\u53ca\u5229\u7528\u53cd\u8f49\u9304-\u5373\u6642\u805a\u5408\u9176\u93c8\u53cd\u61c9(quantitative reverse transcription PCR, qRT-PCR)\u5075\u6e2cEV 71 \u6838\u9178 (RNA)\u3002\u672c\u7814\u7a76\u4ea6\u5229\u7528 SARS-CoV-2 \u6aa2\u6e2c\u6240\u7528\u7684\u4e09\u5c0d\u57fa\u56e0\u7247\u6bb5 (E gene\u3001RdRp gene \u53ca N gene) \u4ee5\u5982\u540c EV 71 \u5728VTM\/I-VTM \u7684\u6e2c\u8a66\u689d\u4ef6\u4e0b\u9032\u884c\u6e2c\u8a66 \u7d50\u679c\u986f\u793a (i) VTM \u5728 4C\u3001-80C\u53ca\u5ba4\u6eab\u7684\u8caf\u5b58\u6307\u5b9a\u6642\u9593\u9ede\u7686\u80fd\u5b8c\u6574\u4fdd\u5b58 EV 71 \u4e4b\u6d3b\u6027 \u800c\u5728RD cell \u7522\u751f CPE (ii) I-VTM \u5728 EV 71 \u63a5\u89f8 2 \u5c0f\u6642\u5f8c\u53ef\u5c07 106 PFU \u7684\u75c5\u6bd2\u5b8c\u5168\u88c2\u89e3\u800c\u5c07\u75c5\u6bd2\u53bb\u6d3b\u5316 (\u6ec5\u6d3b) (iii) VTM \u53ca I-VTM \u5728\u5404\u7a2e\u6e2c\u8a66\u689d\u4ef6\u4e0b\u7686\u80fd\u4fdd\u5b58 EV 71 \u6838\u9178\u53ca SARS- CoV-2 \u4e4b\u6838\u9178\u800c\u5206\u5225\u53ef\u88abqRT-PCR \u65b9\u6cd5\u6aa2\u51fa\u3002\u57fa\u65bc\u4e0a\u8ff0\u7684\u767c\u73fe \u543e\u7b49\u8a8d\u70baVTM \u53caI-VTM \u9069\u5408\u7528\u65bc\u75c5\u6bd2\u6aa2\u9ad4\u7684\u904b\u9001 \u500b\u5225\u6aa2\u9a57\u5ba4\u53ef\u6839\u64da\u75c5\u6bd2\u7684\u6aa2\u9a57\u65b9\u6cd5\u3001\u6aa2\u9a57\u5ba4\u8a2d\u65bd\u7684\u5b89\u5168\u7b49\u7d1a\u3001\u6aa2\u9a57\u6a19\u7684\u75c5\u6bd2\u7684\u98a8\u96aa\u7b49\u7d1a(risk group)\u7b49 \u64c7\u4fdd\u5b58\u578b\u6216\u53bb\u6d3b\u5316\u578b\u75c5\u6bd2\u6aa2\u9ad4\u904b\u9001\u88dd\u7f6e\u8f14\u52a9\u5404\u7a2e\u75c5\u539f\u7684\u6aa2\u6e2c\u3002<\/p>\n

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\u5168\u6587\u8acb\u9ede\u6b64\u4e0b\u8f09<\/p>\n

\u4fdd\u5b58\u578b\u8207\u53bb\u6d3b\u5316\u578b\u75c5\u6bd2\u6aa2\u9ad4\u904b\u9001\u57f9\u990a\u57fa\u7684\u6548\u80fd\u8a55\u4f30-cmp<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\u75c5\u6bd2\u6aa2\u9ad4\u904b\u9001\u57f9\u990a\u57fa(transport media)\u7684\u4e3b\u8981\u529f\u80fd\u662f\u4fdd\u5b58\u6aa2\u9ad4\u4e2d\u75c5\u539f\u9ad4\u7684\u6d3b\u6027\u3001\u6297\u539f\u53ca\u6838\u9178 \u5176\u53ef\u907f\u514d\u6aa2 … Continue reading<\/a><\/p>\n","protected":false},"author":1,"featured_media":14396,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_angie_page":false,"page_builder":"","footnotes":""},"categories":[44],"tags":[462],"class_list":["post-14389","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-hot-news","tag-462"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.cmp-micro.com\/ch\/wp-json\/wp\/v2\/posts\/14389","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.cmp-micro.com\/ch\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.cmp-micro.com\/ch\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.cmp-micro.com\/ch\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.cmp-micro.com\/ch\/wp-json\/wp\/v2\/comments?post=14389"}],"version-history":[{"count":1,"href":"https:\/\/www.cmp-micro.com\/ch\/wp-json\/wp\/v2\/posts\/14389\/revisions"}],"predecessor-version":[{"id":24086,"href":"https:\/\/www.cmp-micro.com\/ch\/wp-json\/wp\/v2\/posts\/14389\/revisions\/24086"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.cmp-micro.com\/ch\/wp-json\/wp\/v2\/media\/14396"}],"wp:attachment":[{"href":"https:\/\/www.cmp-micro.com\/ch\/wp-json\/wp\/v2\/media?parent=14389"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.cmp-micro.com\/ch\/wp-json\/wp\/v2\/categories?post=14389"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.cmp-micro.com\/ch\/wp-json\/wp\/v2\/tags?post=14389"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}