賽多利斯慢病毒純化套組Sartorius Vivapure® LentiSELECT

01 Product Description

賽多利斯慢病毒純化套組Sartorius Vivapure® LentiSELECT

慢病毒(Lentivirus)載體系統亦是現今常見的轉殖技術之ㄧ。其傳統的純化方式與腺病毒載體純化相同,採用超高速離心法,但通常要耗費數十小時以上。而 Vivapure® LentiSELECT Kit將膜層析技術與試劑結合,在1小時之內可將 VSV-G pseudotyped Lentivirus 自 HEK293 細胞株中回收 8 x 108 – 5 x 109 之病毒數。

Fast and easy to use kits for Lentivirus purification The Vivapure® LentiSELECT 40 is a ready-to-use kit for purifying up to 8 x 108 viral particles from 40 ml cell culture. This easy-to-use product replaces time consuming ultracentrifugation methods. Like the AdenoPACK™ kits, this kit includes all reagents and devices necessary for purification and concentration of VSV-G pseudotyped Lentivirus from HEK293 cell culture supern atants in under 1 hours. Optimal recoveries are achieved due to an innovative membrane chromatography assembly, making the use of pre-filters for this configuration needless configuration obsolete.

系列產品:Vivapure® LentiSELECT 40 / Vivapure® LentiSELECT 500 / Vivapure® LentiSELECT 1000.

 

賽多利斯慢病毒純化套組Sartorius Vivapure® LentiSELECT產品列表

套件規格LentiSELECT 40
品名內容
樣本大小40 mL慢病毒上清液
恢復的病毒顆粒(VP)通常最多8 x 108
處理時間通常在1個小時之內
內毒素水平<0.025 EU / mL
套件規格LentiSELECT 500
品名內容
樣本大小500毫升慢病毒上清液
恢復的病毒顆粒(VP)通常最多2-5x 109
處理時間通常在3個小時之內
內毒素水平<0.025 EU / mL
套件規格AdenoPACK™500
品名內容
樣本大小1000毫升慢病毒上清液
恢復的病毒顆粒(VP)通常最多4-5 x 109
處理時間通常在6個小時之內
內毒素水平<0.025 EU / mL