賽多利斯腺病毒純化套組Sartorius Vivapure® AdenoPACK™

01 Product Description

賽多利斯腺病毒純化套組Sartorius Vivapure® AdenoPACK™

重組腺病毒載體(Recombinant adenovirus vectors)是目前效率最好的基因轉殖系統之一,然而傳統的 CsCl 載體回收法費時而冗長,Vivapure® AdenoPACK™ Kit 則採用最新的濾膜技術與試劑系統,將 digestion、virus binding、washing、elution、concentration 等步驟在 2 小時之內執行完畢,最多可於 HEK293 細胞中回收達3 x 1013 的病毒量,回收率為 65-80%,最終體積1 ml。

Fast and Easy to use Kits for Adenovirus Purification Recombinant adenovirus vectors are the preferred method for a wide range of gene delivery applications. The AdenoPACK™ adenovirus purification and concentration kits offer researchers who need to recover up to 3 x 1013 purified recombinant adenovirus particles for in-vitro transfection a fast, safe and easy to use solution. The kits include all reagents and devices necessary for clarification, purification and concentration of adenovirus type 5 from HEK293 cell cultures in only two hours. These straight forward kits replace time-consuming and labor-intensive 48 hour CsCl density gradients.

系列產品:AdenoPACK™ 20 / AdenoPACK™ 100 / AdenoPACK™ 500

 

賽多利斯腺病毒純化套組Sartorius Vivapure® AdenoPACK™產品列表

純化方法處理時間洗脫液復甦病毒顆粒
AdenoPACK TM 20 / 20ml培養物中1小時1 mL65-70%1 x 1011-12
AdenoPACK™ 100 / 60 ml culture1-2小時1 mL65%1-3x1012
AdenoPACK™ 100 / 200 ml culture2小時1 mL80%1 x 1013
AdenoPACK™ 100 RT ***/ 200 ml culture2小時1 mL80%1 x 1013
AdenoPACK™ 500 / 500 ml culture1-2小時1 mL80%1-3x1013
套件規格AdenoPACK™ 20
樣本大小最多6×20毫升的腺病毒上清液---
恢復的病毒顆粒(VP)通常每20 ml製劑最多1 x 1012---
VP/IU20 - 50---
處理時間通常為1小時---
內毒素水平<0.025 EU / mL---
Kit Specifications (AdenoPACK™ 100)
樣本大小20至200毫升的腺病毒上清液---
恢復的病毒顆粒(VP)通常每20 ml製劑最多1 x 1013---
VP/IU20 - 50---
處理時間通常為2小時---
內毒素水平<0.025 EU / mL---
Kit Specifications (AdenoPACK™ 500)
樣本大小多達500毫升的腺病毒上清液---
恢復的病毒顆粒(VP)通常每20 ml製劑最多3 x 1013---
VP/IU20 - 50---
處理時間通常為2小時---
內毒素水平<0.025 EU / mL---